Initial Research On Creatine for Lumbriculus Variegatus Experiment

I have completed my research on the treatment subject for my experiment, lumbriculus variegatus. My lab group has chosen creatine (C4H9N3O2) as our substance to test on the annelid. Our hypothesis is that a proper solution of creatine will increase the worm's pulsation rate. In order to prove this we have to come with a proper ratio of miligrams(mg) to mililiters(mL) so to not overdose the worm and kill it, or underdose the worm and the solution have little or no effect on its pulsation rate. Here is some of the websites that we have conducted research on so far:

Absolute Creatine

All You Need to Know About Creatine

What You Need to Know About Creatine